研究成果

專利：

US 6,777,247 Nervous necrosis virus protein ( 美國專利 , 效期 2004-2024, 已技轉 )
227114 水生動物用口服疫苗 ( 中華民國專利 , 效期 : 2005-2022, 已技轉 )
US 6,872,386 Oral vaccines ( 美國專利 , 效期 : 2005-2025, 已技轉 )

期刊論文：

Yun-Ru Chiang, Ya-Yun Lu, Han-You Lin, 2024, Quantitative Analysis of infectious precocity virus load and stunted growth syndrome via an immunohistochemical assay and reverse transcriptase quantitative real-time PCR in Macrobrachium rosenbergii ; Aquaculture.
Yu-Chuan Chiu, Min-Chi Yeh, Chun-Hsiung Wang, Yu-An Chen, Hsiang Chang, Han-You Lin, Meng-Chiao Ho, Shih-Ming Lin, 2023,Structural basis for calcium-stimulating pore formation of Vibrio α-hemolysin ; Nature Communications.
Meng-Cheng Lai, Hao-Yu Cheng , Sin-Hong Lew, Yu-An Chen, Chien-Hung Yu, Han-You Lin, Shih-Ming Lin, 2023,Crystal structures of dimeric and heptameric mtHsp60 reveal the mechanism of chaperonin inactivation;Life Science Alliance.
Han Tso Lin, Lih Chiann Wang, Yun Ru Chiang, Yi Fan Fang, Fu I. Lu, John Han You Lin,2022,Bioactivity of orange-spotted grouper (Epinephelus coioides) interleukin-6 in innate immunity: Inducing inflammation, antimicrobial peptides, and innate immune molecular gene expression as well as activating phagocytosis of leukocytes and increasing survival under Vibrio and NNV infection;Aquaculture Reports.

Yun-Ru Chiang, Lih-Chiann Wang, Han-Tso Lin, John Han-You Lin,2022,Bioactivity of orange-spotted grouper (Epinephelus coioides) cathepsin L : Proteolysis of bacteria and regulation of the innate immune response;Fish & Shellfish Immunology.
Hsieh-Hsun Ho, Chung-Lun Lu, Pei-Shan Hsieh, Ching-Wei Chen, Shih-Hung Hsieh, Yi-Wei Kuo, Chen-Ruei Liu, Zun-Jie Syu, Jui-Fen Chen, John Han-You Lin,2021,Lactic acid bacteria metabolites in fish feed additives inhibit potential aquatic and food safety pathogens growth, and improve feed conversion;Journal of Applied Aquaculture.
Yo-Wen Wang, Han-You Lin, Jane-Fang Yu, Lih-Chiann Wang,2021,BACTERIAL IDENTIFICATION AND ANTIBIOTIC SENSITIVITY FROM THE ABSCESSES OF CAPTIVE TORTOISES-CLINICAL ANTIBIOTIC RECOMMENDATIONS;Journal of Zoo and Wildlife Medicine.
Shao-Chieh Yen, Ju-Yi Mao, Hung-Yun Lin, Huai-Ting Huang, Scott G. Harroun, Amit Nain, Huan-Tsung, Han-You Lin, Li-Li Chen, Chih-Ching Huang, Han-Jia Lin,2021,Multifunctional carbonized nanogels to treat lethal acute hepatopancreatic necrosis disease;Journal of Nanobiotechnology.
Ding-Li Chou, Ju-Yi Mao, Anisha Anand, Han-Jia Lin, John Han-You Lin, Ching-Ping Tsend, Chih-Ching Huang, Hsian-Yu Wang,2021,Carbonized Lysine-Nanogels Protect against Infectious Bronchitis Virus;International Journal of Molecular Sciences.
Shou-Kuan Tsai, Cheng-Hsin Shih, Hui-Wen Chang, Kuang-Huan Teng, Wei-En Hsu, Han-Jia Lin, Han-You Lin, Ching-Huei Huang,Hui-Wen Chen, Lih-Chiann Wang,2020,Replication of a Dog-Origin H6N1 Inﬂuenza Virus in Cell Culture and Mice；Viruses.
Hung-Yun Lin, Wei-Yu Yeh, Sheng-Fang Tsai, Kuo-Ping Chiang, John Han-You Lin, Che-Chia Tsao, Han-Jia Lin,2020,Biological Protective Effects Against Vibrio Infections in Grouper Larvae Using the Strombidium sp. NTOU1, a Marine Ciliate Amenable for Scaled-Up Culture and With an Excellent Bacteriovorous Ability；Frontier in Marine Science.
Huai-Ting Huang, Han-Jia Lin, Hui-Ju Huang, Chih-Ching Huang, John Han-You Lin, Li-Li Chen,2020,Synthesis and evaluation of polyamine carbon quantum dots (CQDs) in Litopenaeus vannamei as a therapeutic agent against WSSV；Scientific reports.
Shou-Kuan Tsai, Chen-Chih Chen, Han-Jia Lin, Han-You Lin, Ting-Chih Chen, Lih-Chiann Wang,2019,Combination of multiplex reverse transcription recombinase polymerase amplification assay and capillary electrophoresis provides high sensitive and high-throughput simultaneous detection of avian influenza virus subtypes；Journal of Veterinary Science.
JJ Lee,HY Lin,CA Chen,CS Lin,LC Wang ,2019,Development of an oligonucleotide microarray for simultaneous detection of two canine MDR1 genotypes and association between genotypes and chemotherapy side effects；Journal of Veterinary Science.
Hou, Chia Yi; Lin, John Han You; Lin, Shih Jie; Kuo, Wan Ching; Lin, Han Tso,2016,Down-regulation of CD53 expression in Epinephelus coioides under LPS, poly (I:C), and cytokine stimulation,Fish and Shellfish Immunology; 魚類與甲殼類免疫學,51,pp143-152 (SCI)
Ho, Li Ping; Chang, Chin Jun; Liu, Hsiao Cheng; Yang, Huey Lang; Lin, John Han You,2014,Evaluating the protective efficacy of antigen combinations against Photobacterium damselae ssp. piscicida infections in cobia, Rachycentron canadum L ；魚類疾病期刊,Journal of Fish Disesase;,37,1,pp51-62 (SCI)
Hsu, Yi Jiou; Hou, Chia Yi; Lin, Shih Jie; Kuo, Wan Ching; Lin, Han Tso; Lin, John Han You,2013,The biofunction of orange-spotted grouper (Epinephelus coioides) CC chemokine ligand 4 (CCL4) in innate and adaptive immunity,Fish and Shellfish immunology; 魚類與甲殼類免疫學,35,6,pp1891-1898 (SCI)
Chen, H.H.; Lin, H.T.; Foung, Y.F.; Lin, J.H.Y,2012,The bioactivity of teleost IL-6: IL-6 protein in orange-spotted grouper (Epinephelus coioides) induces Th2 cell differentiation pathway and antibody production,Developmental and comparative immunology;,38,2,pp285-294 (SCI)
Yeh, Y.C.; Lee, C.W.; Pan, Y.W.; Hsu, Y.J.; Hung, H.Y.; Chen, Y.M.; Lin, H.Y.; Chen, T.Y.; Yang, H.L.; Wang, H.C.,2012,Identification and characterization of DSCAM isoforms isolated from Orange-Spotted Grouper Epinephelus coioides,Developmental and comparative immunology;,38,1,pp148-159 (SCI)
Chen, Tsung-Yuan; Lin, Han-You; Lin, Chin-Chiu; Lu, Chung-Kuan; Chen, Yi-Min,2012,Picochorum as an alternative to Nannochloropsis for grouper larval rearing,Aquaculture;,338-341,pp82-88 (SCI)
Chen, Yi Min; Shih, Ching Hung; Liu, Hsiao Chien; Wu, Cheng Lung; Lin, Chin Chiu; Wang, Han Ching; Chen, Tzong Yueh; Yang, Huey Lang,2011,An oral nervous necrosis virus vaccine using Vibrio anguillarum as an expression host provides early protection,Aquaculture;, (SCI)
Liu, Fu-Chien; Su, Chung-Ren; Wu, Tzi-Yi; Su, Shyh-Gang; Yang, Huey-Lang; Lin, Han-You; Wu, Tian-Shung,2011,Efficient (1)H-NMR quantitation and investigation of N-acetyl-D-glucosamine (GlcNAc) and N,N'-diacetylchitobiose (GlcNAc)(2) from chitin,International Journal of Molecular Sciences;,12,pp5828-5841 (SCI)
Lam, Wai San; Wu, Szu Yin; Lin, Shih Jie; Lin, Chin Chiu; Chen, Yi Min; Wang, Han Ching; Chen, Tzong Yueh; Lin, Han Tso,2011,The expression of two novel orange-spotted grouper (Epinephelus coioides) TNF genes in peripheral blood leukocytes, various organs, and fish larvae,Fish and Shellfish Immunology;,30,pp618-629 (SCI)
Ho, Li Ping; Lin, Han You; Liu, Hsiao Chien; Chen, Huey En; Chen, Tzong Yueh,2011,Identification of antigens for the development of a subunit vaccine against Photobacterium damselae ssp. piscicida,Fish and Shellfish Immunology;,30,pp412-419 (SCI)
KC Han-Ching Wang; Chun-Wei Tseng; Han-You Lin; I-Tung Chen; Ya-Hui Chen; Yi-Min Chen; Tzong-Yueh Chen; Huey-Lang Yang,2010,RNAi knock-down of the Litopenaeus vannamei Toll gene (LvToll) significantly increases mortality and reduces bacterial clearance after challenge with Vibrio harveyi.,Developmental and comparative immunology;,34,1,pp49-58 (SCI)
Chang C-C; Lin P-S; Hou M-Y; Lin C-C; Hung M-N; Wu T-M; Shu P-Y; Shin W-Y; Lin J H-Y; Chen W-C; Wu H-S; Lin L-J,2009,Identification of Risk Factors of Coxiella burnetii (Q fever) Infection in Veterinary-Associated Populations in Southern Taiwan.,Zoonoses and public health;, (SCI)
Pin-Hsiang Chou; Hao-Shuo Chang; I.-Tung Chen; Han-You Lin; Yi-Min Chen; Huey-Lang Yang; K.C. Han-Ching Wang,2009,The putative invertebrate adaptive immune protein Litopenaeus vannamei Dscam (LvDscam) is the first reported Dscam to lack a transmembrane domain and cytoplasmic tail.,Developmental and comparative immunology;,33,12,pp1258-67 (SCI)
John Han-You Lin; Han-Tso Lin; Carmen Lopez; Tzong-Yueh Chen; Ming-Shyan Chen; Huey-LangYang,2008,A Comparison of the Expression of immunity-related rag-1 and ikaros Genes with Histogenesis of the Thymus in Epinephelus malabaricus.,Aquaculture Research;,39,pp252-262 (SCI)
Young-Mao Chen; Yung-Lin Su; Pei-Shiuan Shie; John Han-You Lin; Huey-Lang Yang; Tzong-Yueh Chen,2008,Grouper Mx confers resistance to nodavirus and interacts with coat protein.,Developmental and comparative immunology;,32,7,pp825-836 (SCI)
*CC Lin; JHY Lin ; MS Chen ; HL Yang,2007, An oral Nervous Necrosis Virus vaccine that induces protective immunity in larvae of grouper ( Epinephelus coioides) ,Aquaculture,268,pp265-273 (SCI)
*JHY Lin; TY Chen; MS Chen; HE Chen; RL; Chou; ZY Cheng; MS Su ; HL Yang,2006,Vaccination with three inactivated pathogens of cobia ( Rachycentron canadum ) stimulates protective immunity,Aquaculture,255,1-4,pp125-132 (SCI)
*SP Chen; HL Yang; GM Her; HY Lin ; MF Jeng; JL Wu ; JR Hong,2006,Betanodavirus induces phosphatidylserine exposure and loss of mitochondrial membrane potential in secondary necrotic cells, both of which are blocked by bongkrekic acid ,Virology,347,pp379-391 (SCI)
*YM Chen; YL Su; JHY Lin ; HL Yang ; TY Chen,2006,Cloning of an orange-spotted grouper (Epinephelus coioides) Mx cDNA and characterisation of its expression in response to nodavirus ,Fish and Shellfish Immunology,20,1,pp58-71 (SCI)
*SP Chen; HL Yang; HY Lin ; MC Chen; JL Wu ; JR Hong,2006, Enhanced viability of a nervous necrosis virus-infected stable cell line over-expressing a fusion product of the zfBl-xL and green fluorescent protein genes,Journal of Fish Diseases,29,pp347-354 (SCI)
*HT Lin; HY Lin ; HL Yang,2005, Histology and histochemical enzyme-staining patterns of major immune organs in Epinephelus malabaricus,Journal of Fish Biology,66,3,pp729-740 (SCI)
JHY Lin ; CC Yu; CC Lin ; HL Yang,2005,An oral delivery system for recombinant subunit vaccine to fish.,Developments in biologicals;,121,pp175-180 (期刊論文)
*PR Rajan; JHY Lin ; MS Ho ; Yang HL,2003, Simple and rapid detection of Photobacterium damselae subsp. piscida by a PCR technique and plating method,Journal of Applied Microbiology,95,pp1375-1380 (SCI)

研討會論文：

J.H.Y. Lin, C.C. Lin, S.J. Lin, W.C. Kuo, Y.F. Foung, H.T. Lin,2014,The external administrated fish cytokine will increase the survival in bacterial and virus challenge.
J.H.Y. Lin, C.C. Lin, S.J. Lin, W.C. Kuo, Y.F. Foung, H.T. Lin,2014,Fish Cytokines Could Use as A Disease APrevention or Treatment Reagent.
John Han-You Lin ,2013,Immuneprophelaxis Strategy for Nodavirus Prevention in grouper (Epinephelus spp.) larvae.
H.H. Chen, H.T. Lin, Y.F. Foung, J.H.Y. Lin,2012,The bioactivity of teleost IL-6: orange-spotted grouper (Epinephelus coioides) IL-6 induce Th2 cell differentiation pathway and antibody production.
Y.J. Hsu, C.Y. Ho, J.H.Y. Lin ,2012,Using Chemokine Gene Expression As A Biomarker For Disease Infection.
*CH Shih; HC Liu; CL Wu; CC Lin; YM Chen; HY Lin ; HL Yang,2008, Vibrio expression system: a novel host for nervous necrosis virus subunit vaccine production,;,Taipei , Taiwan,
HY Lin; YM Chen; PF Wu; JY Chen; HL Yang,2008, Development a gene-deleted subunit vaccine of Aeromonas hydrophila,World Aquaculture Symposium 2008;,Busan , Korea,
Chen Y-M; Shie P-S; Yang H-L; Chen T-Y,2008,Roles of grouper Mx in regulation of cell cycle and resistance to nodavirus.,The 7th Symposium on Diseases in Asian Aquaculture.;,Taipei, Taiwan.,
Chen Y-M; Shie H-P; Yang H-L; Chen T-Y,2008,Identification of small heat shock protein as stress-induced molecular chaperone-like activity in nodavirus infected grouper.,The 7th Symposium on Diseases in Asian Aquaculture.;,Taipei, Taiwan.,
Tasi T-J; Lee S-S; Chen Y-M; Lee G-B,; Yang H-L; Chen T-Y,2008,One step multiplex diagnosis for grouper pathogens detection.,The 7th Symposium on Diseases in Asian Aquaculture.;,Taipei, Taiwan.,
Chang C-W; Chen L-J; Su Y-C; Yang, H-L; Wu J-L; Hong J-R,2008,Identification of a novel antiapoptotic protein B1 encoded by the RGNNV TN1 strain in fish cells.,The 7th Symposium on Diseases in Asian Aquaculture.;,Taipei, Taiwan.,
*HL Yang; CM Lu; CC Lin; MS Chen ; JHY Lin,2007,Improve Protection Against White Spot Syndrome Virus (WSSV) of Tiger Shrimp Penaeus monodon by Oral Administration of Artemia Encapsulation Inactivated Vibrio ,Stirling , Scotland,
*HC Chuang; YM Chen; HY Lin ; HL Yang ; TY Chen,2006,Detection of Nerve Necrosis Virus by a Real-time PCR Method and Characterization of Its Tissue Distributions in Giant Grouper (Epinephelus lanceolatus), Hiroshima, Japan,
*HL Yang; HY Lin ; CC Lin,2006,The development of an oral NNV vaccine and its efficacy evaluation in the larvae of Epinephelus coioides, Hiroshima , Japan,
*HY Lin; YM Chen; MS Chen; CC Lin ; HL Yang,2006, Mornitoring nervous necrosis virus infection in a commercil grouper hatchery in Taiwan, Hiroshima,Japan,
*CC Lin; MS Chen; HY Lin ; HL Yang,2006, Establishment of experimental challenge model of grouper ( Epinephelus coioides ) for NNV, Hiroshima,Japan,

研究計畫：

應用多胺加工物替代抗生素開發新型家禽與豬飼料添加劑產品 [2021]
探討髓鞘過程對神經發育、退化與再生的影響--利用斑馬魚及石斑魚探討髓鞘再形成及其在神經系統再生之功能 [共同主持人, 2020~2022]
以多胺碳量子點做為魚類養殖抗生素替代物 [2019/08/01~2020/07/31]
石斑魚 IL-6與受器反應及Hyper IL-6 在非特異性免疫反應以及抗菌功能評估 [2018/08/01~2019/07/31]
價創計畫：精炭粒(SC-Dot)新型態安全高效除菌劑 [共同主持人, 2017~2018]
點帶石斑魚TNF-α1及TNF-α2之間的交互作用，以及不同組成對於免疫系統的影響[2017/08/01~2018/07/31]
石斑魚二腫瘤壞死因子α(TNF-α1/α2)在非特異性免疫反應所扮演角色之研究 [2015/08/01 ~ 2016/07/31]
魚類IL-6蛋白在疾病防治上的應用 [2012~ 2013]
萌芽個案計畫-提高石斑魚產業養殖過程存活率之新策略-研發神經壞死病毒及細菌性感染疾病控制之新技術[2012~ 2013]
石斑魚細菌及病毒合併之多價型注射疫苗之研究 [2012]
優質石斑魚培育養殖鏈整合研究--〈子計畫二〉應用基因體所篩檢的免疫分子應用於魚類疾病防治[2012]
石斑魚腫瘤壞死因子基因選殖與其在魚苗發展階段基因表現及功能性研究 [2010/08/01 ~ 2011/07/31]
石斑魚腫瘤壞死因子基因選殖與其在魚苗發展階段基因表現及功能性研究 [2009/08/01 ~ 2010/07/31]
白蝦免疫相關基因Astakine之選殖與功能探討 [共同主持人, 2009]
跨領域新技術商品化，產學合作及創業平台之研究 [共同主持人, 2009~2010]
以轉錄體學方式分析石斑魚免疫細胞之免疫相關基因及其表現 [2009/08/01 ~ 2010/07/31]
建立點帶石斑魚重組TNF-α蛋白分子表現及其功能性分析 [2009/07/01 ~ 2010/02/28]
石斑魚腫瘤壞死因子基因選殖與其在魚苗發展階段基因表現及功能性研究 [2008/08/01 ~ 2009/07/31]
海鱺細菌多價口服疫苗研發 [2007]

專書著作：

1. 郭宛靜、林翰佑、林翰佳、徐嫚君、賴佩欣, 2021, ⟪養蝦，你會不會忘了什麼？⟫, 養魚世界出版。
2. JHY Lin , CC Yu, CC Lin and HL Yang, 2005, An oral delivery system for recombinant subunit vaccine to fish, Mydtlyng (ed): Fish Vaccinology, Dev Biol. Basel Karger 121:175-180